What does 1 kb ladder mean?

Product Description. 1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 – 10,000 base pairs (bp). The ladder consists of 13 double-stranded, blunt-end fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1).

What is Gene ruler?

Description. GeneRuler DNA Ladders are universal ladders used for sizing and approximation of DNA bands. Covering a wide range of DNA fragments (100 bp-1 kb), the ladders are provided in ready-to-use formats and contain TriTrack loading buffer for easy gel loading applications.

How much DNA ladder should I load?

We recommend loading 10 μl (0.5 μg) of Quick-Load 1 kb DNA Ladder per gel lane.

What is a 100 bp ladder?

Invitrogen 100 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 2,000 bp. 100 bp DNA Ladder consists of 13 individual chromatography-purified DNA fragments and has reference bands at 2000, 1500, and 600 bp for easy orientation.

What is a 1 kb DNA size standard ladder?

The 1 kb DNA ladder is a unique combination of a number of plasmids digested with restriction enzymes and PCR products to yield 13 DNA fragments that are suitable for use as a molecular weight standard for electrophoresis.

How do you store a DNA ladder?

Storage, Stability and Shipment PCR DNA ladder may be kept safely at room temperature for at least 3 months from date of shipment. Ladders are guaranteed for 12 months when stored at 4°C. Aliquot product if necessary to avoid repeated freezing and thawing cycles. Shipped at ambient temperature.

Where on the gel will the largest DNA molecules be and why?

The largest fragments are near the top of the gel (negative electrode, where they began), and the smallest fragments are near the bottom (positive electrode).

How do you dilute 100 bp ladder?

This ladder is preferred by our lab because of its broad range (100 bp to 10,000 bp) and even intervals. Its stock concentration is 1 µg/µl of DNA. The ladder is diluted to a 1:4 solution in water for use (3 parts water for 1 part ladder). To make 100 µl, 75 µl of water are combined with 25 µl of the DNA ladder.

Can you dilute DNA Ladder?

Dilute only 1 µl of DNA Ladder at a time *For multiple loads, dilution, and storage, use TE or other buffer of minimal ionic strength instead of water. DNA may denature if diluted and stored in dH20. This protocol is recommended for a 5mm wide gel lane.